Annual Reports on NMR Spectroscopy: 62


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Recommend to Librarian. By irradiating and observing at twice the 14 N Larmor frequency, overtone OT nuclear magnetic resonance NMR is capable of obtaining 14 N OT spectra without first-order quadrupolar broadening. A recent study [L. Ratcliffe, Chem. This article has also shown that under MAS the main 14 N OT peak is shifted by twice the sample spinning frequency with respect to its static position. We present the theory of 14 N OT NMR of static or rotating samples and the physical picture of the intriguing spinning-induced shift in the second case.

We use perturbation theory for the case of static samples and Floquet theory for rotating samples. In both cases, the results can be described by a so-called OT parameter that scales down the 14 N OT radio-frequency rf excitation and signal detection. This OT parameter shows that the components of the rf field, which are transverse and longitudinal with respect to the magnetic field, are both effective for 14 N OT rf excitation and signal detection.

This leads to an apparent 14 N OT signal shifted at twice the spinning frequency. The spinning induced shift and its dependence on the spinning direction are confirmed experimentally by reversing the spinning direction and the field of the 36 T series-connected hybrid magnet at the US National High Magnetic Field Laboratory. Find more information on the Altmetric Attention Score and how the score is calculated.

The information from a small number of plane projections is used to recreate the full multidimensional spectrum in the familiar format. Projections at any desired angle of incidence are obtained by Fourier transformation of time-domain signals acquired when two or more evolution intervals are incremented simultaneously at different rates.

The new technique relies on an established Fourier transform theorem that relates time-domain sections to frequency-domain projections.

NMR Spectroscopy

Recent developments in NMR instrumentation, such as increased resolution and sensitivity, make fast methods for data gathering much more practical for protein and RNA research. A new reconstruction algorithm is proposed, based on the inverse Radon transform. Examples are presented of three- and four-dimensional NMR spectra of nuclease A inhibitor reconstructed by this technique with significant savings in measurement time.

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Annual Reports on NMR Spectroscopy

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Annual Reports on NMR Spectroscopy: 62 Annual Reports on NMR Spectroscopy: 62
Annual Reports on NMR Spectroscopy: 62 Annual Reports on NMR Spectroscopy: 62
Annual Reports on NMR Spectroscopy: 62 Annual Reports on NMR Spectroscopy: 62
Annual Reports on NMR Spectroscopy: 62 Annual Reports on NMR Spectroscopy: 62
Annual Reports on NMR Spectroscopy: 62 Annual Reports on NMR Spectroscopy: 62

Related Annual Reports on NMR Spectroscopy: 62



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